Poly (N-isopropylacrylamide)-functionalized dendrimer as a thermosensitive nanoplatform for delivering malloapelta B against HepG2 cancer cell proliferation
1. Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi, Ben Nghe Ward, Ho Chi Minh City, Vietnam
2. Department of Biotechnology, Ton Duc Thang University, 19 Nguyen Huu Tho, Tan Phong Ward, District 7, Ho Chi Minh City, Vietnam
3. Institute of Organic Chemistry with Centre of Phytochemistry, Acad. G. Bonchev Str., bl. 9 BG-1113 Sofia City, Bulgaria
4. Institute of Marine Biochemistry, 18 Hoang Quoc Viet, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Tóm tắt
In recent years, nanocarriers have emerged as effective platforms for delivering several kinds of herbal medicine and naturally bioactive compounds. In this study we developed an outstanding thermosensitive dendritic nanocarrier to efficiently deliver malloapelta B (Mall B), which is a water insoluble bioactive compound isolated from leaves of Mallotus apelta—Vietnamese medicinal plant. The thermosensitive poly(N-isopropylacrylamide) (PNIPAM) polymer-conjugated polyamidoamine (PAMAM) dendrimer copolymer was prepared via Michael reaction. The copolymer structures were confirmed by proton nuclear magnectic resonance (1H NMR). Morphology of the nanocarrier was observered around 70–120 nm by transmission electron microscopy (TEM). Size distributions were measured by dynamic light scattering (DLS) of the nanocarrier and its Mall B-loaded performed at 146.8 nm and 194.5 nm, respectively. The PNIPAM-g-PAMAM-based nanocarrier exhibited higher Mall B loading efficiency (DL = 59.93 ± 0.19%) and entrapment efficiency (EE = 89.98 ± 2.06%) as compared to PNIPAM (DL = 52.54 ± 0.45% and EE = 66.45 ± 2.78%). In vitro release indicated that approximately 30% amount of the loaded Mall B released at pH 5.5 after 54 h tracking. At the same time, 12.5% amount of the molecules released at pH 7.4.Cytotoxicity assay results showed that the Mall B-loaded nanocarrier significantly inhibited HepG2 cancer cell proliferation. These obtained results indicated that the nanocarrier could solve hydrophobic property of Mall B for further medicine applications.
Tài liệu tham khảo
Lipinski C A 2000 J. Pharmacol. Toxicol. Methods 44 235
Williams H D, Trevaskis N L, Charman S A, Shanker R M, Charman W N, Pouton C W and Porter C J 2013 Pharmacol. Rev. 65 315
Vieth M, Siegel M G, Higgs R E, Watson I A, Robertson D H, Savin K A, Durst G L and Hipskind P A 2004 J. Med. Chem. 47 224
Sakthi U M, Lobo F J R and Uppuluri K B 2013 Biomed. Pharmacol. J. 6 355
Fahr A and Liu X 2007 Expert Opin. Drug Deliv. 4 403
Wong S M, Kellaway I W and Murdan S 2006 Int. J. Pharm. 317 61
Rabinow B E 2004 Nat. Rev. Drug Discov. 3 785
Ghebremeskel A N, Vemavarapu C and Lodaya M 2007 Int. J. Pharm. 328 119
Srinarong P, Kouwen S, Visser M R, Hinrichs W L and Frijlink H W 2010 Pharm. Dev. Technol. 15 460
Serajuddin A T 2007 Adv. Drug. Deliv. Rev. 59 603
Park J W, Kim S H and Choi S J 2016 Appl. Biol. Chem. 59 471
Choi J H, Joung Y K, Bae J W, Choi J W, Tran N Q and Park K D 2011 Macromol. Res. 19 180
Wongpinyochit T, Uhlmann P, Urquhart A J and Seib F P 2015 Biomacromolecules 16 3712
Fonseca C, Simões S and Gaspar R 2002 J. Control. Release 83 273
Tong N A, Nguyen T P, Nguyen C K and Tran N Q 2016 J. Biomater. Sci. Polym. Ed. 27 709
Kim S S, Rait A, Kim E, DeMarco J, Pirollo K F and Chang E H 2015 Cancer. Lett. 369 250
Nguyen H, Nguyen N H, Tran N Q and Nguyen C K 2014 J. Nanosci. Nanotechnol. 15 4106
Massing U and Fuxius S 2000 Drug Resist. Update 3 171
Nguyen T T C, Nguyen C K, Nguyen T H and Tran N Q 2016 Mater. Sci. Eng. C 70 992
Ly T U, Tran N Q, Hoang T K, Phan K N, Truong H N and Nguyen C K 2013 J. Biomed. Nanotechnol. 9 213
Shi C Y, Guo D D, Xiao K, Wang X, Wang L L and Luo J T 2015 Nat. Commun. 6 7449
Tran N Q, Nguyen C K and Nguyen T P 2013 Adv. Nat. Sci: Nanosci. Nanotechnol. 4 045013
Nabid M R, Rezaei S J T, Sedghi R, Niknejad H, Entezami A A, Oskooie H A and Heravi M M 2011 Polymer 52 2799
Sun P, Zhang Y, Shi L and Gan Z 2010 Macromol. Biosci. 10 621
Wang Z, Zhou B, Liu D and Fan X P 2010 Macromol. Symp. 297 158
Gerber B, Scholz C, Reimer T, Briese V and Janni W 2006 Breast Cancer Res. Treat. 95 199
Tsai C H, Lin F M, Yang Y C, Lee M T, Cha T L, Wu G J, Hsieh S C and Hsiao P W 2009 Clin. Cancer Res. 15 5435
Liang H L M, Xue C C L, Zhou D H and Li C G 2011 Chin. J. Integr. Med. 9 157
Stickel F and Schuppan D 2007 Dig. Liver. Dis. 39 293
Luu V C, Chau V M, Lee J J and Jung S H 2006 Arch. Pharm. Res. 29 840
Ma J, Shi H, Mi C, Li H L, Lee J J and Jin X 2015 Int. Immunopharmacol. 24 147
Yadav D, Suri S, Choudhary A A, Sikender M, Hemant, Beg N M, Garg V, Ahmad A and Asif M 2011 Int. J. Pharm. Tech. 3 3092
Bisht S, Feldmann G, Soni S, Ravi R, Karikar C, Maitra A and Maitra A 2007 J. Nanobiol. 5 1
Mathur M and Vyas G 2013 Indian J. Nat. Prod. Resour. 4 329
Le P N, Nguyen N H, Nguyen C K and Tran N Q 2016 Bull. Mater. Sci. 39 1493
Ankrum J A, Miranda O R, Sng K, Sarkar D, Xu C J and Karp J M 2014 Nat. Protocols 9 233
Papazisis K T, Geromichalos G D, Dimitriadis K A and Kortsaris A H 1997 J. Immunol. Methods 208 151
Vichai V and Kirtikara K 2011 Nat. Protocols 1 1112
Williams H D, Trevaskis N L, Charman S A, Shanker R M, Charman W N, Pouton C W and Porter C J 2013 Pharmacol. Rev. 65 315
Vieth M, Siegel M G, Higgs R E, Watson I A, Robertson D H, Savin K A, Durst G L and Hipskind P A 2004 J. Med. Chem. 47 224
Sakthi U M, Lobo F J R and Uppuluri K B 2013 Biomed. Pharmacol. J. 6 355
Fahr A and Liu X 2007 Expert Opin. Drug Deliv. 4 403
Wong S M, Kellaway I W and Murdan S 2006 Int. J. Pharm. 317 61
Rabinow B E 2004 Nat. Rev. Drug Discov. 3 785
Ghebremeskel A N, Vemavarapu C and Lodaya M 2007 Int. J. Pharm. 328 119
Srinarong P, Kouwen S, Visser M R, Hinrichs W L and Frijlink H W 2010 Pharm. Dev. Technol. 15 460
Serajuddin A T 2007 Adv. Drug. Deliv. Rev. 59 603
Park J W, Kim S H and Choi S J 2016 Appl. Biol. Chem. 59 471
Choi J H, Joung Y K, Bae J W, Choi J W, Tran N Q and Park K D 2011 Macromol. Res. 19 180
Wongpinyochit T, Uhlmann P, Urquhart A J and Seib F P 2015 Biomacromolecules 16 3712
Fonseca C, Simões S and Gaspar R 2002 J. Control. Release 83 273
Tong N A, Nguyen T P, Nguyen C K and Tran N Q 2016 J. Biomater. Sci. Polym. Ed. 27 709
Kim S S, Rait A, Kim E, DeMarco J, Pirollo K F and Chang E H 2015 Cancer. Lett. 369 250
Nguyen H, Nguyen N H, Tran N Q and Nguyen C K 2014 J. Nanosci. Nanotechnol. 15 4106
Massing U and Fuxius S 2000 Drug Resist. Update 3 171
Nguyen T T C, Nguyen C K, Nguyen T H and Tran N Q 2016 Mater. Sci. Eng. C 70 992
Ly T U, Tran N Q, Hoang T K, Phan K N, Truong H N and Nguyen C K 2013 J. Biomed. Nanotechnol. 9 213
Shi C Y, Guo D D, Xiao K, Wang X, Wang L L and Luo J T 2015 Nat. Commun. 6 7449
Tran N Q, Nguyen C K and Nguyen T P 2013 Adv. Nat. Sci: Nanosci. Nanotechnol. 4 045013
Nabid M R, Rezaei S J T, Sedghi R, Niknejad H, Entezami A A, Oskooie H A and Heravi M M 2011 Polymer 52 2799
Sun P, Zhang Y, Shi L and Gan Z 2010 Macromol. Biosci. 10 621
Wang Z, Zhou B, Liu D and Fan X P 2010 Macromol. Symp. 297 158
Gerber B, Scholz C, Reimer T, Briese V and Janni W 2006 Breast Cancer Res. Treat. 95 199
Tsai C H, Lin F M, Yang Y C, Lee M T, Cha T L, Wu G J, Hsieh S C and Hsiao P W 2009 Clin. Cancer Res. 15 5435
Liang H L M, Xue C C L, Zhou D H and Li C G 2011 Chin. J. Integr. Med. 9 157
Stickel F and Schuppan D 2007 Dig. Liver. Dis. 39 293
Luu V C, Chau V M, Lee J J and Jung S H 2006 Arch. Pharm. Res. 29 840
Ma J, Shi H, Mi C, Li H L, Lee J J and Jin X 2015 Int. Immunopharmacol. 24 147
Yadav D, Suri S, Choudhary A A, Sikender M, Hemant, Beg N M, Garg V, Ahmad A and Asif M 2011 Int. J. Pharm. Tech. 3 3092
Bisht S, Feldmann G, Soni S, Ravi R, Karikar C, Maitra A and Maitra A 2007 J. Nanobiol. 5 1
Mathur M and Vyas G 2013 Indian J. Nat. Prod. Resour. 4 329
Le P N, Nguyen N H, Nguyen C K and Tran N Q 2016 Bull. Mater. Sci. 39 1493
Ankrum J A, Miranda O R, Sng K, Sarkar D, Xu C J and Karp J M 2014 Nat. Protocols 9 233
Papazisis K T, Geromichalos G D, Dimitriadis K A and Kortsaris A H 1997 J. Immunol. Methods 208 151
Vichai V and Kirtikara K 2011 Nat. Protocols 1 1112
Các bài trích dẫn đến
1.
Jun Li, Huamin Liang, Jing Liu, Ziyuan Wang.
Poly (amidoamine)(PAMAM) dendrimer mediated delivery of drug and pDNA/siRNA for cancer therapy
in International journal of pharmaceutics (2018)
2.
Phung Ngan Le, Chan Khon Huynh, Ngoc Quyen Tran.
Advances in thermosensitive polymer-grafted platforms for biomedical applications
in Materials Science and Engineering: C (Vol. 92, 2018)
3.
Vikrant Saluja, Abhinav Mankoo, Gaurav K Saraogi, Murtaza M Tambuwala, Vijay Mishra.
Smart dendrimers: Synergizing the targeting of anticancer bioactives
in Journal of Drug Delivery Science and Technology (2019)