Graphene patterned polyaniline-based biosensor for glucose detection

    Hai Binh Nguyen 1
    Van Chuc Nguyen 1
    Van Tu Nguyen 1
    Thi Thanh Tam Ngo 1
    Ngoc Thinh Nguyen 2
    Thi Thu Huyen Dang 3
    Dai Lam Tran 1
    Phuc Quan Do 4
    Xuan Nghia Nguyen 1
    Xuan Phuc Nguyen 1
    Hong Khoi Phan 1
    Ngoc Minh Phan 1

    Advances in Natural Sciences: Nanoscience and Nanotechnology - Vol. 3, No. 2 (2012)

    1. Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam
    2. School of Chemical Engineering, Hanoi University of Science and Technology 1, Dai Co Viet Road, Hanoi, Vietnam
    3. Faculty of Chemistry, Hanoi Pedagogical University, No 2 Nguyen Van Linh Road, Xuan Hoa, Phuc Yen District, Vinh Phuc, Vietnam
    4. Research Center for Environmental Technology and Sustainable Development, Hanoi University of Science, Vietnam National University Hanoi, 334 Nguyen Trai Road, Hanoi, Vietnam

    Tiếng Anh

    Tóm tắt

    This paper describes a glucose electrochemical biosensor, layer-by-layer fabricated from graphene and polyaniline films. Graphene sheets (0.5 × 0.5 cm2 ) with the thickness of 5 nm (15 layers) were synthesized by thermal chemical vapor deposition (CVD) under ambient pressure on copper tapes. Then they were transferred into integrated Fe3O4-doped polyaniline (PANi) based microelectrodes. The properties of the nanocomposite films were thoroughly characterized by scanning electron microscopy (SEM), Raman spectroscopy, atomic force microscopy (AFM) and electrochemical methods, such as square wave voltametry (SWV) and chronoamperometry. The above graphene patterned sensor (denoted as Graphene/Fe3O4/PANi/GOx) shows much improved glucose sensitivity (as high as 47µA mM−1 cm−2 ) compared to a non-graphene one (10–30µA mM−1 cm−2 , as previously reported in the literature). It can be expected that this proof-of-concept biosensor could be extended for other highly sensitive biodetection.

    Từ khoá

    Keywords: graphene, glucose, electrochemical biosensor, layer-by-layer, polyaniline (PANi)

    Tài liệu tham khảo

    [1] Pumera M, Sanchez S, Ichinose I and Tang J 2007 Sensors Actuators B 123 1195

    [2] Pearson J E, Gill A and Vadgama P 2000 Ann. Clin. Biochem. 37 119

    [3] Bartlett P N and Cooper J M 1993 J. Electroanal. Chem. 362 1

    [4] Sotiropoulou S, Vamvakaki V and Chaniotakis N A 2005 Biosensors Bioelectron. 20 1674

    [5] López B P and Merkoçi A 2011 Trends Food Sci. Technol. 22 625

    [6] Balasubramanian K and Burghard M 2006 Anal. Biochem. 385 452

    [7] Pumera M, Ambrosi A, Bonanni A, Chung E L K and Poh H L 2010 Trends Anal. Chem. 29 954

    [8] Lu C H, Yang H H, Zhu C L, Chen X and Chen G N 2009 Angew. Chem., Int. Ed. Engl. 48 4785

    [9] Feng L, Chen Y, Ren J and Gu X 2011 Biomaterials 32 2930

    [10] Potts J R, Dreyer D R, Bielawski C W and Ruoff B S 2011 Polymer 52 5

    [11] Luong T T et al 2011 Coll. Surf. A 384 23

    [12] Tran L D, Nguyen D T, Nguyen B H, Do Q P and Nguyen H L 2011 Talanta 85 1560

    [13] Li X et al 2009 Science 324 1312

    [14] Kim K S, Zhao Y, Jang H, Lee S Y, Kim J M, Kim K S, Ahn J H, Kim P, Choi J Y and Hong B H 2009 Nature 457 706

    [15] Gao L, Guest J R and Guisinger N P 2010 Nano Lett. 10 3512

    [16] Yang L, Ren X, Tang F and Zhang L 2009 Biosensors Bioelectron. 25 889

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